II. Restriction fragment length polymorphisms.

III. Variable number of tandem repeats.

IV. Single nucleotide polymorphisms.

V. Gene therapy.

VI. Transgenic organisms.

Terms.

A. In order to be useful as a marker, the inherited variations must be polymorphic, i.e. there must be two or more common variations in the population under study. Monomorphic sites/genes have only one common form and therefore are not useful as markers.

B. A genetic marker can be a functional gene. However, it is not the function that is of interest but the variations in DNA structure that are reflected in functional differences between alleles. For example, blood groups can be used in gene mapping or to resolve issues of identity, paternity, or who committed a crime. The biological function of blood groups is not related to these applications.

C. A genetic marker can also be a segment of DNA that has no known function or that is known not to have a function. So long as that DNA varies among homologous chromosomes, it can serve as a genetic marker that is transmitted according to Mendelian rules for codominant alleles.

D. There are some dozens of polymorphic functional genes that are useful as markers. By contrast, there are thousands of polymorphic DNA sites that are useful as markers.

A. Some one per thousand base pairs (bp = nucleotide pairs) varies in the population, i.e. instead of an AT pair, there might be a TA, GC, or CG pair. Often these are polymorphic.

B. Some of these variants involve a sequence susceptible to attack by a restriction enzyme. The DNA of one sequence may be cut by a certain restriction enzyme; the other sequence is not a target for this restriction enzyme and is not cut. Since there will be flanking restriction sites, the first form will generate two DNA fragments; the second only one.

C. The fragments can be separated on the basis of size by means of electrophoresis and detected in Southern blots.

D. The "trait" that is expressed is variation in length of fragments of DNA (restriction fragments); thus the name restriction fragment length polymorphism. Most restriction sites are not polymorphic, however.

E. These DNA variations are transmitted strictly by Mendelian rules as codominant traits and make excellent genetic markers.

A. There are many DNA sequences of unknown function that are repeated hundreds or even hundreds of thousands of times in the genome. Some are typically scattered throughout the genome; others are repeated in tandem and may occur at only a few sites.

B. At a tandem repeat site, the number of repeats varies widely in the population, although the repeat number is usually well preserved during transmission. Therefore each different repeat number can be treated as a separate "allele" and the site can be treated as a highly polymorphic site with multiple alleles. Such a site is known as a VNTR (variable number of tandem repeats) site

C. Minisatellites have a repeating unit of 100-200 nt.

1. A restriction enzyme that cleaves on either side of the VNTR region generates fragments that differ in length.

2. These "alleles" are detected by Southern blots, using probes that bind to the repeating unit.

D. Microsatellites have repeating units of 2-4 nt.
1. Because the overall length of the VNTR region is small, PCR can be used to amplify the entire region by selecting primers that flank the VNTR region.

2. The resulting fragments are separated by electrophoresis and do not require a probe for detection.

V. The techniques developed for gene cloning are the basis for most approaches to gene therapy.

A. Functional versions of the defective genes are cloned into viral vectors that introduce the desired gene into cells of the affected person.

B. In ex vivo procedures, cells (usually bone marrow cells) of the patient are removed and infected with a vector into which has been inserted the functional gene. The modified cells are injected back into the patient, where they are encouraged in various ways to flourish. This approach is useful for conditions in which the primary defect is in the lymphocytes or is in blood metabolites that are in contact with the lymphocytes. E.g. ADA in severe combined immunodeficiency.

C. In in vivo procedures, the viral vector is introduced into the patient, where it invades target cells, carrying along the correct version of the gene. E.g. adenovirus vector and CFTR, the gene that is defective in cystic fibrosis.

A. In plants, genes are incorporated into a virus called Ti that can infect plants in general and that integrates into and is transmitted as part of the host DNA. E.g. FlavrSavr tomatoes.

B. In mice, DNA is injected directly into a zygote, which is then implanted into a foster mother. During the early cell divisions, the injected DNA frequently is incorporated into chromosomes and becomes part of the genome of the descendent cells. The mouse that develops from that zygote is a chimera, i.e. some of the cells are modified and some are not. However, individual gametes produced by that mouse either have the new DNA or they do not. Therefore, some offspring of that mouse will come from zygotes that are modified, and the offspring will not be chimeras.

C. In knockout mice, the DNA that is injected into the zygote is engineered so that it is inserted in the homologous site in the host DNA, causing the host gene to become nonfunctional. In this way, mice can be bred that have specific genes mutated to nonfunctional forms. Many recessive genes of great interest in humans have been "knocked out" in mice, creating the mouse equivalents of human diseases. E.g. cystic fibrosis.

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