The University of Texas at Austin
  • Busted! Virus Caught Infecting Cell

    By Lee Clippard
    Published: Jan. 16, 2013

    video still from video showing how a virus infects a cell

    Watch the video depiction of a T7 virus “walking” across a cell.

    The detailed changes in the structure of a virus as it infects an E. coli bacterium have been observed for the first time, report researchers from The University of Texas at Austin and The University of Texas Health Science Center at Houston (UT Health) Medical School this week in Science.

    To infect a cell, a virus must be able to first find a suitable cell and then eject its genetic material into its host. This robot-like process has been observed in a virus called T7 and visualized by Ian Molineux, professor of biology in the College of Natural Sciences at The University of Texas at Austin, and his colleagues.

    The researchers show that when searching for its prey, the virus briefly extends — like feelers — one or two of six ultra-thin fibers it normally keeps folded at the base of its head.

    Once a suitable host has been located, the virus behaves a bit like a planetary rover, extending these fibers to walk randomly across the surface of the cell and find an optimal site for infection.

    At the preferred infection site, the virus goes through a major change in structure in which it ejects some of its proteins through the bacterium’s cell membrane, creating a path for the virus’s genetic material to enter the host.

    After the viral DNA has been ejected, the protein path collapses and the infected cell membrane reseals.

    “Although many of these details are specific to T7,” said Molineux, “the overall process completely changes our understanding of how a virus infects a cell.”

    For example, the researchers now know that most of the fibers are usually bound to the virus head rather than extended, as was previously thought. That those fibers are in a dynamic equilibrium between bound and extended states is also new.

    Molineux said that the idea that phages “walk” over the cell surface was previously proposed, but their paper provides the first experimental evidence that this is the case.

    This is also the first time that scientists have made actual images showing how the virus’s tail extends into the host — the very action that allows it to infect a cell with its DNA.

    “I first hypothesized that T7 made an extended tail more than 10 years ago,” said Molineux, “but this is the first irrefutable experimental evidence for the idea and provides the first images of what it looks like.”

    The researchers used a combination of genetics and cryo-electron tomography to image the infection process. Cryo-electron tomography is a process similar to a CT scan, but it is scaled to study objects with a diameter a thousandth the thickness of a human hair.

    Molineux’s co-authors are Bo Hu, William Margolin and Jun Liu from UT Health.

    This article originally appeared in Texas Science.

    • Quote 2
      bell said on Jan. 19, 2013 at 10:00 a.m.
      so if a virus has to find that perfect cell in a sense.whu no drug to alter a cell enough to be like a ghost to the virus.which makes the virus unable to spread..common colds would be a relief
    • Quote 2
      Joshua said on Jan. 18, 2013 at 12:21 p.m.
      I cannot access the video right now so I don't know if you cover this. What hardware was used and what imaging software? It is great to be able to visualize reactions at that scale!
    • Quote 2
      Sarah said on Jan. 17, 2013 at 5:42 p.m.
    • Quote 2
      Bill said on Jan. 16, 2013 at 11:15 a.m.
      What can you say but WOW! This is terrific science and hopefully will lead to protecting us from harmful viruses.
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