Publications

Whitman, C.P. "The 4-Oxalocrotonate Tautomerase Family of Enzymes: How Nature Makes New Enzymes Using a β-α-β Structural Motif" Arch. Biochem. Biophys. 402, 1-13 (2002).

Wang, S.C, Person, M.D., Johnson, Jr., W.H., and Whitman, C.P. "Reactions of trans-3-Chloroacrylic Acid Dehalogenase with Acetylene Substrates: Consequences of and Evidence for a Hydration Reaction " Biochemistry 42, 8762-8773 (2003).

Poelarends, G.J., Johnson, Jr., W.H., Murzin, A.G., and Whitman, C.P. "Mechanistic Characterization of a Bacterial Malonate Semialdehyde Decarboxylase: Identification of a New Activity in the Tautomerase Superfamily" J. Biol.Chem. 278, 48674-48683 (2003).

Poelarends, G.J., Serrano, H., Person, M.D., Johnson, Jr., W.H., Murzin, A.G., and Whitman, C.P. "Cloning, Expression, and Characterization of a cis-3-Chloroacrylic Acid Dehalogenase: Insights into the Mechanistic, Structural, and Evolutionary Relationship between Isomer-specific 3-Chloroacrylic Acid Dehalogenases" Biochemistry 43, 759-772 (2004).

de Jong, R.M., Brugman, W., Poelarends, G.J., Whitman, C.P., and Dijkstra, B.W. "The X-ray Structure of trans-3-Chloroacrylic Acid Dehalogenase Reveals a Novel Hydration Mechanism in the Tautomerase Superfamily" J. Biol.Chem. 279, 11546-11552 (2004).

Azurmendi, H.F., Wang,S.C., Massiah, M.A., Poelarends, G.J., Whitman, C.P. and Mildvan, A.S. "The Roles of Active-site Residues in the Catalytic Mechanism of trans-3-Chloroacrylic Acid Dehalogenase: A Kinetic, NMR, and Mutational Analysis", Biochemistry 43, 4082-4091 (2004).

Poelarends, G.J., Serrano, H., Johnson, Jr., W.H., and Whitman, C.P. "Stereospecific Alkylation of cis-Chloroacrylic Acid Dehalogenase by (R)-Oxirane-2-carboxylate: Analysis and Mechanistic Implications", Biochemistry 43, 7187-7196 (2004).

Johnson, Jr., W.H., Wang, S.C, Stanley, T.M., Czerwinski, R.M, Almrud, J.J., Murzin, A.G., and Whitman, C.P. "4-Oxalocrotonate Tautomerase, its Homologue YwhB, and Active Vinylpyruvate Hydratase: Synthesis and Evaluation of Fluoride Substrate Analogs" Biochemistry, (in press).

Poelarends, G.J. and Whitman, C.P. "Evolution of Enzymatic Activity in the Tautomerase Superfamily: Mechanistic and Structural Studies of the 1,3-Dichloropropene Catabolic Enzymes", Bioorganic Chem, (in press).