The Analytical Instrumentation Facility Core (CRED FC2) provides services and collaborative research for the detection, characterization, and quantification of biomolecules via HPLC and mass spectrometry instrumentation. The lab in PHR 1.110 (College of Pharmacy) houses analytical HPLC-EC, GC-MS, LC-ESI-ion trap, LC-ESI-QTRAP, nanoflow HPLC-MALDI, and MALDI-TOF instruments, while a MALDI-TOF/TOF instrument is located in MBB 1.420 (ICMB). The Analytical Core is supported by the Center for Research on Environmental Disease, NIEHS center grant ES007784, and by institutional funds provided by ICMB, UT-Austin, MDACC-Science Park, and the College of Pharmacy.
News
Kevin Shores will be conducting a training session on the MALDI-TOF on June 11 at 2 pm in PHR 1.110.
Maria Person will be giving a talk at the NIH Workshop on Efficient Management and Utilization of Core Facilities on July 14-15. The workshop will be videocasted.
Core Services and Collaborative Projects:
Protein:
Protein identification
Protein/peptide molecular weight determination
Peptide MS/MS fragmentation for sequence determination
Solution digest of proteins
Separation of proteins/peptides by HPLC
Immobilized Metal Affinity Chromatography enrichment of phosphopeptides
Characterization of protein modifications (phosphorylation, acetylation, symmetric/asymmetric methylation, covalent adduct, inhibitor binding sites)
Identification of N-linked glycopeptides
Characterization of protein fragments, isoforms, and variants
Protein-ligand binding stoichiometry
Relative quantitation of stable isotope labeled peptides
Small Bio-molecules/DNA/Chemicals:
8-oxo-deoxyguanosine from extracted DNA to 30 fmol by HPLC-EC
neurotransmitters to 50 fmol: dopamine, DOPAC, deoxyepinephrine, 5-HIAA, serotonin and norepinephrine from dialysate by HPLC-EC
Homocysteine and cysteine to 1 pg from plasma/serum by GC-MS
Homocysteine to 100 pg, S-amino ethyl cysteine, folic acid to 1 pg, methyl tetrahydrofolic acid (MHFA) to 10 pg, and vitamin B12 to 1 pg by QTRAP
Fatty acids, prostaglandins, isoprostanes from lysates by GC-MS
Fatty acid methyl ester (FAME) by GC-MS
Glutathione derivatized hydroquinone identification/quantification by HPLC-EC; LC-MS
Arginine/Citrulline relative abundance determination by LCQ
4-aminobiphenyl to 0.1 pg by GC-MS
4-hydroxynonenal to 10 pg by GC-MS
Glutathione-4-hydroxynonenal to 0.5 pg by QTRAP
Dicarboxylic acids (Citric, Fumaric, Malonic, Succinic) by GC-MS
Purine, Pyrimidine derivatives mustard (Cl-ethyl-ethyl sulfide, Cl-ethyl-methyl sulfide, mechlorethamine) adducts by LCQ and GC
Saxitoxin to 4 pmol by LCQ
Anandaminde to 0.5 pg and 2-arachidonylglycerol to 50 pg by QTRAP
Fluconzaole and voriconazole to 5 pg by QTRAP
C12 and C18 homoserine lactone to 5 pg by QTRAP
Extraction of hydrophilic and hydrophobic metabolites from tissues and biofluids
Quantification of unlisted drugs, nutrients, natural products, and xenobiotics may be tested and performed by HPLC-UV/Fluor/EC, GC-MS, or QTRAP
We have partnerships with the ICMB Protein Facility Core and the CRED FC1 Molecular Biology Core which provide gel based protein services such as in-gel digest.
Maria D. Person, Ph.D.
Director
Stony H. Lo, Ph.D.,
Manager
Kevin S. Shores, Ph.D.,
Research Associate
Jacqulyn Tolson, Laboratory
Assistant
Mailing Address:
CRED Analytical Facility Core
College of Pharmacy
The University of Texas
at Austin
1 University Station
A1900
Austin, TX, USA
78712-01200
Email Address: mperson
@mail.utexas.edu
Phone:
(512)232-4340
