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Maria Person, Ph.D., Director
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The Analytical Instrumentation Facility Core (FC2) provides services for the characterization of chemicals and biomolecules via HPLC and mass spectrometry instrumentation. The lab in PHR 1.110 (College of Pharmacy) houses analytical HPLC, GC-MS, LC-ESI-ion trap, and MALDI-TOF instruments, while the LC-MALDI and MALDI-TOF/TOF instruments are located in MBB 1.420 (ICMB). The Analytical Core is supported by the Center for Research on Environmental Disease, NIEHS center grant ES07784, and by institutional funds provided by ICMB and the College of Pharmacy.
News
The 4000 QTRAP hybrid triple quadrupole-linear ion trap is the latest acquisition. Installed last fall, it has high sensitivity and extended linear dynamic range and is an excellent choice for quantitative analysis. Lab testing of metabolites, hormones, and nutrients has found limits of detection in the range of 1-100 pg, and currently it is being used to quantify serum components.
Core Services and Collaborative Projects:
Protein:
Protein identification
Protein/peptide molecular weight determination
Peptide MS/MS fragmentation for sequence determination
Immobilized Metal Affinity Chromatography enrichment of phosphopeptides
Characterization of protein modifications (phosphorylation, acetylation, symmetric/asymmetric methylation, covalent adduct, inhibitor binding sites)
Identification of protein-protein cross-link sites
Characterization of protein isoforms/variants
Protein-ligand binding stoichiometry
Separation of proteins/peptides by 1D and 2D-HPLC
Small Bio-molecules/DNA/Chemicals:
Quantification of 8-oxo-deoxyguanosine from extracted DNA to 30 fmol by HPLC-EC
Quantification of neurotransmitters to 50 fmol: dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC), deoxyepinephrine, 5-hydroxyindole-3-acetic acid, serotonin and norepinephrine from cells/brain dialysate by HPLC-EC
Homocysteine and cysteine quantification from plasma/serum by GC-MS
Fatty acid/prostaglandins, isoprostanes from cells lysates, extraction/ derivatization/ quantification by GC-MS
Fatty acid methyl ester (FAME) identification and quantification by GC-MS
Glutathione derivatized hydroquinone identification/quantification by HPLC-EC; LC-MS
Arginine/Citrulline relative abundance determination by LC-MS
4-hydroxynonenal and 4-aminobiphenyl quantification by GC-MS
Dicarboxylic acids (Citric, Fumaric, Malonic, Succinic acid) derivatization/quantification by GC-MS
Acetylated galactose identification by GC-MS
Purine, Pyrimidine derivatives mustard (Cl-ethyl-ethyl sulfide, Cl-ethyl-methyl sulfide, mechlorethamine) adducts identification by LC-MS
Mustard (Cl-ethyl-ethyl sulfide)/hydrolyzed product (Ethyl-2-hydroxyl-ethyl-sulfide) identification by GC-MS
Quantification of unlisted general drugs, nutrients, natural products, and chemicals may be tested and performed by HPLC-UV/Fluor/EC, LC-MS or GC-MS