The Analytical Instrumentation Facility Core (CRED FC2) provides services and collaborative research for the detection, characterization, and quantification of biomolecules via HPLC and mass spectrometry instrumentation. The lab in PHR 1.110 (College of Pharmacy) houses analytical HPLC-EC, GC-MS, LC-ESI-ion trap, LC-ESI-QTRAP, nanoflow HPLC-MALDI, and MALDI-TOF instruments, while a MALDI-TOF/TOF instrument is located in MBB 1.420 (ICMB). The Analytical Core is supported by the Center for Research on Environmental Disease, NIEHS center grant ES007784, and by institutional funds provided by ICMB, UT-Austin, MDACC-Science Park, and the College of Pharmacy.
Core Services and Collaborative Projects:
Protein Identification, Characterization and Quantitation:
Protein identification
Protein/peptide molecular weight determination
Peptide MS/MS fragmentation for sequence determination
Separation of proteins/peptides by HPLC
Immobilized Metal Affinity Chromatography enrichment of phosphopeptides
Characterization of protein modifications (phosphorylation, acetylation, symmetric/asymmetric methylation, covalent adduct, inhibitor binding sites)
Identification of N-linked glycopeptides
Characterization of protein fragments, isoforms, and variants
Protein-ligand binding stoichiometry
Relative quantitation of stable isotope labeled peptides with iTRAQ
Metabolites/Nutrients/DNA/Drugs Detection and Quantitation:
QTRAP:
Prostaglandins: PGD2 – 0.5pg, PGE2 – 1pg, PGF2α – 10pg, PGJ2 – 5pg
Hydroxy-eicosatetraenoic acid (HETE): 5-HETE – 5pg, 12-HETE – 5pg, 15-HETE – 5pg
Leukotriene B4 (LTB4) –0.5pg, Leukotriene C4 (LTC4) –10pg
Steroids: Androstenedione – 5pg, Estradiol – 100pg, Pregnenolone – 2ng, Progesterone – 50pg, Testosterone – 20pg
Tocopherols: α –Tocopherol – 0.5ng, ϒ –Tocopherol – 2ng ,α –TEA – 10ng, ϒ –TEA – 20ng
2-hydroxy propylphosphonic acid (2HPP) – 5pg
Simvastatin – 0.5pg, Simvastatin Acid – 5 pg
Homocysteine – 100 pg, folic acid – 1 pg
methyl tetrahydrofolic acid (MHFA) – 10 pg
vitamin B12 – 1 pg
Glutathione-4-hydroxynonenal – 0.5 pg
Anandaminde – 0.5 pg, 2-arachidonylglycerol – 50 pg
Fluconzaole– 5 pg , Voriconazole – 5 pg
C12 and C18 homoserine lactone – 5 pg
GC-MS:
Homocysteine and cysteine – 1 pg
Fatty acids, prostaglandins, isoprostanes
Fatty acid methyl ester (FAME)
4-aminobiphenyl – 0.1 pg
4-hydroxynonenal – 10 pg
LCQ:
Arginine/Citrulline relative abundance determination
Saxitoxin – 4 pmol
Purine, Pyrimidine derivatives mustard (Cl-ethyl-ethyl sulfide, Cl-ethyl-methyl sulfide, mechlorethamine) adducts
HPLC-EC:
8-oxo-deoxyguanosine from extracted DNA – 30 fmol
Neurotransmitters – 50 fmol: dopamine, DOPAC, deoxyepinephrine, 5-HIAA, serotonin and norepinephrine from dialysate
Glutathione derivatized hydroquinone identification/quantification
Quantification of other compounds may be tested using HPLC-UV/Fluor/EC, GC-MS, or QTRAP
We have partnerships with the ICMB Protein Facility Core and the CRED FC1 Molecular Biology Core which provide gel based protein services such as in-gel digest.
Maria D. Person, Ph.D.
Director
Stony H. Lo, Ph.D.,
Manager
Kevin S. Shores, Ph.D.,
Research Associate
Jacqulyn Tolson, Laboratory
Assistant
Mailing Address:
CRED Analytical Facility Core
College of Pharmacy
The University of Texas
at Austin
1 University Station
A1900
Austin, TX, USA
78712-01200
Email Address: mperson
@mail.utexas.edu
Phone:
(512)232-4340
