The Protein and Metabolite Analysis Facility provides services, self-service equipment, and collaborative research for the detection, characterization, and quantification of biomolecules. The facility labs are located in MBB 1.420 (ICMB) and PHR 1.110 (College of Pharmacy). The facility is supported by the Cancer Prevention Research Institute of Texas Shared Instrumentation Award RP110782 and by institutional funds provided by ICMB, UT-Austin, MDACC-Science Park, and the College of Pharmacy.
News and Announcements:
Mass spectrometry:
Protein/peptide molecular weight determination by ESI or MALDI
Protein identification by LC-MS/MS and database search for individual gel bands and complex mixtures
Relative quantitation of peptides/proteins with spectral counting, peak area, or stable isotope labeling*
Protein modification analysis for phosphorylation, methylation, acetylation, ubiquitinylation, glycosylation, inhibitor binding, and chemical adduct site determination*
Fatty Acid Methyl Ester quantification by GC-MS
Other MBB lab services/instrumentation:
Edman chemistry N-terminal Sequencing
Peptide synthesis
Separation of proteins/peptides by HPLC or FPLC (4°C or room temperature)
IMAC and MOAC enrichment of phosphopeptides
Self-service instrumentation available:
SDS-PAGE
GC-MS (Experienced users only, training not available)
HPLC and FPLC (4°C or room temperature)
Crystallography robot
Luminometer
MALDI-TOF MS molecular weight determination
When using core services, remember to cite the Protein and Metabolite Analysis Facility in your publications, and funding sources ES007784 (CRED) and RP110782 (CPRIT) when appropriate, and send the citation to Maria Person. Continued support for the facility depends on documented outcomes.
*If you are interested in collaborating on a protein modification or quantitation project, contact Maria Person.
Delivery Address: Protein and Metabolite Analysis Facility Email Address:
pmaf Phone: Institute for Cellular and Molecular Biology (ICMB) Center for Research on Environmental Disease (CRED)
Maria D. Person
Director
Michelle V. Gadush
MBB Lab Manager
Marvin Mercado
PHR Lab Manager
Chia-Fang Lee
CPRIT post-doctoral fellow
Jacqulyn Tolson,
Research Assistant
The University of Texas
at Austin
2500 Speedway
Stop A4800
Austin, TX, USA
78712-1639
@austin.utexas.edu
(512)471-2895
