The Protein and Metabolite Analysis Facility provides services, self-service equipment, and collaborative research for the detection, characterization, and quantification of biomolecules. The facility labs are located in MBB 1.420 (ICMB) and PHR 1.110 (College of Pharmacy). The facility is supported by the Cancer Prevention Research Institute of Texas Shared Instrumentation Award PR110782, Center for Research on Environmental Disease, NIEHS center grant ES007784, and by institutional funds provided by ICMB, UT-Austin, MDACC-Science Park, and the College of Pharmacy.
News and Announcements:
Chia-Fang Lee is presenting a lecture on mass spectrometry methods on February 28 from 9-10 am in MBB 2.304.
Marvin Mercado is lab manager for the PHR labs. Please contact him for protein and peptide MW determination services and access to PHR 1.110.
Stony Lo is no longer working at the facility. The metabolite quantitation services are currently suspended, except for GC-MS based fatty acid methyl ester quantitation which is available provisionally through Jacqulyn Tolson.
The LTQ Velos Pro mass spectrometer is being repaired, but the protein identification service is continuing on the Orbitrap Elite.
If you are interested in collaborating on a protein modification or quantitative proteomics project or conducting a cancer research proteomics experiment on the state-of-the-art Orbitrap Elite mass spectrometer, contact Maria Person.
Core Services and Collaborative Projects:Mass spectrometry:
Protein/peptide molecular weight determination by ESI or MALDI
Protein identification by LC-MS/MS and database search for individual gel bands and complex mixtures
Relative quantitation of peptides/proteins with spectral counting or stable isotope labeling
Protein modification analysis for phosphorylation, methylation, acetylation, ubiquitinylation, glycosylation, inhibitor binding, and chemical adduct site determination
Fatty Acid Methyl Ester quantification
Other MBB lab services/instrumentation:
Edman chemistry N-terminal Sequencing
Peptide synthesis
Separation of proteins/peptides by HPLC or FPLC (4°C or room temperature)In-gel and solution proteolytic digest
IMAC and MOAC enrichment of phosphopeptides
Luminex antibody bead based quantitation:
TNF alpha, MCP-1, adiponectin, resistin, insulin, leptin, GM-CSF, Interferon-gamma, IL-1 alpha, IL-1 beta, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12(p70), IL-13, IGF1, PAI-1, mTOR, phosho-mTOR, ERK 1/2, phospo-ERK 1/2, AKT/PKB, phospho-AKT/PKB, p70 S6 kinase, phospho-p70 S6 kinase. New assays can be implemented using kits available from vendors.
Self-service instrumentation/ training available:
SDS-PAGE
Peptide synthesis
HPLC and FPLC (4°C or room temperature)
Crystallography robot
Luminometer
MALDI-TOF MS molecular weight determination
When using core services, remember to cite the Protein and Metabolite Analysis Facility in your publications, and funding sources ES007784 and PR110782 when appropriate, and send the citation to Maria Person or inclusion on our website. Continued support for the facility depends on documented outcomes.
Delivery Address: Protein and Metabolite Analysis Facility Email Address:
pmaf Phone: Institute for Cellular and Molecular Biology (ICMB) Center for Research on Environmental Disease (CRED)
Maria D. Person
Director
Michelle V. Gadush
MBB Lab Manager
Marvin Mercado
PHR Lab Manager
Chia-Fang Lee
CPRIT post-doctoral fellow
Cissy Geigerman
Luminex Specialist
Jacqulyn Tolson,
Research Assistant
The University of Texas
at Austin
2500 Speedway
MBB 1.420
Austin, TX, USA
78712
@austin.utexas.edu
(512)471-2895
