Beckman System Gold HPLC (2)
Amersham Biosciences AKTA FPLC
Biorad BioLogic DuoFlow (2 systems-room temp. and 4 C)
Recent publications using LC systems:
- Self-service LC for purification of biomolecules.
- The DuoFlow system is equipped with a conductivity meter as well as a UV detector along
with a fraction collector, all controlled by a common software platform. QuadTec UV/Vis Detector - simultaneous monitering of four wavelengths allows optimal detection range of 190-740 nm. The fraction collector has an option for collecting fractions at the user's convenience in different formats. Sample volume as little 100 ul, or up to 5 ml using the injection loop. BioLogic Maximizer valve system provides buffer blending function.
Maiya R, Ponomarev I, Linse KD, Harris RA, Mayfield RD. Defining the dopamine transporter proteome by convergent biochemical and in silico analyses. Genes Brain Behav. 2007;6(1):97-106. PMID: 16643512. PMAF Usage: Agilent 1100 HPLC separation of Dopamine transporter associated proteins, Esquire ESI-MS protein MW
Callaway K, Abramczyk O, Martin L, Dalby KN. The anti-apoptotic protein PEA-15 is a tight binding inhibitor of ERK1 and ERK2, which blocks docking interactions at the D-recruitment site. Biochemistry. 2007;46(32):9187-98. PMID: 17658892. PMAF Usage: Symphony peptide synthesis; AKTA HPLC purification
Borisova SA, Liu HW. Characterization of glycosyltransferase DesVII and its auxiliary partner protein DesVIII in the methymycin/picromycin biosynthetic pathway. Biochemistry. 2010;49(37):8071-84. PMCID: 2939310. PMID: 20695498. PMAF Usage: LCQ ESI-MS protein MW, AKTA HPLC purification, Esquire MS
Be'er A, Ariel G, Kalisman O, Helman Y, Sirota-Madi A, Zhang HP, et al. Lethal protein produced in response to competition between sibling bacterial colonies. Proc Natl Acad Sci U S A. 2010;107(14):6258-63. PMCID: 2851949. PMID: 20308591. PMAF Usage: HPLC separation of peptides, Procise N-terminal sequencing identification of peptides
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Last Reviewed: January 18, 2011