Proteomics Facility


MALDI - MS Instrumentation:

PHOTO

PHOTO


AB 4700 Proteomics Analyzer MALDI-TOF/TOF (MBB 1.420)

AB Voyager-DE PRO MALDI-TOF (PHR 1.110)

AB Voyager MALDI-TOF (MBB 1.420)

Applications:

Protein MW determination, unlimited mass range
Peptide and metabolite MW measurements
Peptide MS/MS on 4700
Protein identification of single proteins from MS + MS/MS on 4700 with picomole sensitivity
Self-service use available for trained users on the Voyager instruments

Contact:

Maria Person
Marvin Mercado
Michelle Gadush

Internal Rates:

MS $46/sample
MS-self service $23/sample
Protein identification $80/sample, bill 2x for modification analysis
For external rate, go to charges page.

Links:

View digestion protocol

View Representative Data

Recent publications:

Cullen TW, Giles DK, Wolf LN, Ecobichon C, Boneca IG, Trent MS. Helicobacter pylori versus the Host: Remodeling of the Bacterial Outer Membrane Is Required for Survival in the Gastric Mucosa. PLoS Pathog. 2011;7(12):e1002454. PMID: 22216004 PMAF Usage: MALDI of Lipid A

Lee S, Warthaka M, Yan C, Kaoud TS, Ren P, Dalby KN. Examining docking interactions on ERK2 with modular peptide substrates. Biochemistry. 2011;50(44):9500-10. PMID: 21955038 PMAF Usage: MALDI of peptide MW

Bu Q, Zhu L, Dennis MD, Yu L, Lu SX, Person MD, Tobin EM, Browning KS, Huq E. Phosphorylation by CK2 enhances the rapid light-induced degradation of phytochrome interacting factor 1 in Arabidopsis. J Biol Chem. 2011;286(14):12066-74. PMID: 21330376 PMAF Usage: MALDI phosphorylation analysis

Arroyo LA, Herrera CM, Fernandez L, Hankins JV, Trent MS, Hancock RE. The pmrCAB operon mediates polymyxin resistance in Acinetobacter baumannii ATCC 17978 and clinical isolates through phosphoethanolamine modification of lipid A. Antimicrob Agents Chemother. 2011;55(8):3743-51. PMID: 21646482 PMAF Usage: MALDI of Lipid A

Rowe AH, Xiao Y, Scales J, Linse KD, Rowe MP, Cummins TR, Zakon HH. Isolation and characterization of CvIV4: a pain inducing Ī±-scorpion toxin. PLoS One. 2011;6(8):e23520. PMID: 21887265 PMAF Usage: MALDI of peptide MW

Hankins JV, Madsen JA, Giles DK, Childers BM, Klose KE, Brodbelt JS, Trent MS. Elucidation of a novel Vibrio cholerae lipid A secondary hydroxy-acyltransferase and its role in innate immune recognition. Mol Microbiol. 2011;81(5):1313-29. PMID: 21752109 PMAF Usage: MALDI of Lipid A Lee S, Warthaka M, Yan C, Kaoud TS, Piserchio A, Ghose R, Ren P, Dalby KN. A model of a MAPKā€¢substrate complex in an active conformation: a computational and experimental approach. PLoS One. 2011;6(4):e18594. PMID: 21494553 PMAF Usage: MALDI of peptide MW

Johnson CM, Linsky TW, Yoon DW, Person MD, Fast W. Discovery of halopyridines as quiescent affinity labels: Inactivation of dimethylarginine dimethylaminohydrolase. JACS 2011;133(5):1553-62. PMID: 21222447 PMAF Usage: MALDI identification of inhibitor binding site

Stead CM, Zhao J, Raetz CR, Trent MS. Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface. Mol Microbiol. 2010;78(4):837-52. PMCID: 2978256. PMID: 20659292. PMAF Usage: MALDI of Lipid A

Jain A, Bacolla A, Chakraborty P, Grosse F, Vasquez KM. Human DHX9 helicase unwinds triple-helical DNA structures. Biochemistry. 2010;49(33):6992-9. PMCID: 2949957. PMID: 20669935. PMAF Usage: MALDI protein ID

Herrera CM, Hankins JV, Trent MS. Activation of PmrA inhibits LpxT-dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides. Mol Microbiol. 2010;76(6):1444-60. PMCID: 2904496. PMID: 20384697. PMAF Usage: MALDI of Lipid A

Guo Z, Kozlov S, Lavin MF, Person MD, Paull TT. ATM activation by oxidative stress. Science. 2010;330(6003):517-21. PMID: 20966255. PMAF Usage: targeted LC-MALDI cysteine modification analysis

Gorini G, Ponomareva O, Shores KS, Person MD, Harris RA, Mayfield RD. Dynamin-1 co-associates with native mouse brain BKCa channels: proteomics analysis of synaptic protein complexes. FEBS Lett. 2010;584(5):845-51. PMCID: 2828775. PMID: 20114047. PMAF Usage: MALDI protein ID, in-gel digest

Cullen TW, Trent MS. A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuni. Proc Natl Acad Sci U S A. 2010;107(11):5160-5. PMCID: 2841920. PMID: 20194750. PMAF Usage: MALDI of Lipid A

Alexander A, Cai SL, Kim J, Nanez A, Sahin M, MacLean KH, et al. ATM signals to TSC2 in the cytoplasm to regulate mTORC1 in response to ROS. Proc Natl Acad Sci U S A. 2010;107(9):4153-8. PMCID: 2840158. PMID: 20160076. PMAF Usage: IMAC phosphopeptide enrichment, MALDI and targeted LC-MALDI phosphopeptide analysis

Stead CM, Zhao J, Raetz CR, Trent MS. Removal of the outer Kdo from Helicobacter pylori lipopolysaccharide and its impact on the bacterial surface. Mol Microbiol. 2010;78(4):837-52. PMCID: 2978256. PMID: 20659292. PMAF Usage: MALDI of Lipid A

Herrera CM, Hankins JV, Trent MS. Activation of PmrA inhibits LpxT-dependent phosphorylation of lipid A promoting resistance to antimicrobial peptides. Mol Microbiol. 2010;76(6):1444-60. PMCID: 2904496. PMID: 20384697. PMAF Usage: MALDI of Lipid A

Cullen TW, Trent MS. A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuni. Proc Natl Acad Sci U S A. 2010;107(11):5160-5. PMCID: 2841920. PMID: 20194750. PMAF Usage: MALDI of Lipid A

Zhang Z, Stevens LM, Stein D. Sulfation of eggshell components by Pipe defines dorsal-ventral polarity in the Drosophila embryo. Curr Biol. 2009;19(14):1200-5. PMCID: 2733793. PMID: 19540119. PMAF Usage: MALDI protein ID

Umeda A, Thibodeaux GN, Zhu J, Lee Y, Zhang ZJ. Site-specific protein cross-linking with genetically incorporated 3,4-dihydroxy-L-phenylalanine. Chembiochem. 2009;10(8):1302-4. PMCID: 2752657. PMID: 19422009. PMAF Usage: MALDI protein ID

Jeter CR, Badeaux M, Choy G, Chandra D, Patrawala L, Liu C, et al. Functional evidence that the self-renewal gene NANOG regulates human tumor development. Stem Cells. 2009;27(5):993-1005. PMID: 19415763. PMAF Usage: MALDI protein ID

Hankins JV, Trent MS. Secondary acylation of Vibrio cholerae lipopolysaccharide requires phosphorylation of Kdo. J Biol Chem. 2009;284(38):25804-12. PMCID: 2757982. PMID: 19617350. PMAF Usage: MALDI of Lipid A

Dennis MD, Person MD, Browning KS. Phosphorylation of plant translation initiation factors by CK2 enhances the in vitro interaction of multifactor complex components. J Biol Chem. 2009;284(31):20615-28. PMCID: 2742826. PMID: 19509420. PMAF Usage: IMAC phosphopeptide enrichment, MALDI phosphopeptide analysis

Dennis MD, Browning KS. Differential phosphorylation of plant translation initiation factors by Arabidopsis thaliana CK2 holoenzymes. J Biol Chem. 2009;284(31):20602-14. PMCID: 2742825. PMID: 19509278. PMAF Usage: MALDI protein ID

Yan M, Shen J, Person MD, Kuang X, Lynn WS, Atlas D, et al. Endoplasmic reticulum stress and unfolded protein response in Atm-deficient thymocytes and thymic lymphoma cells are attributable to oxidative stress. Neoplasia. 2008;10(2):160-7. PMCID: 2244691. PMID: 18283338. PMAF Usage: MALDI protein ID

Robertson BA, Johnson WH, Lo HH, Jr., Whitman CP. Inactivation of Cg10062, a cis-3-chloroacrylic acid dehalogenase homologue in Corynebacterium glutamicum, by (R)- and (S)-oxirane-2-carboxylate: analysis and implications. Biochemistry. 2008;47(33):8796-803. PMCID: 2646761. PMID: 18646866. PMAF Usage: MALDI for inhibitor binding site determination

Poelarends GJ, Serrano H, Person MD, Johnson WH, Jr., Whitman CP. Characterization of Cg10062 from Corynebacterium glutamicum: implications for the evolution of cis-3-chloroacrylic acid dehalogenase activity in the tautomerase superfamily. Biochemistry. 2008;47(31):8139-47. PMCID: 2659011. PMID: 18598055. PMAF Usage: MALDI characterization of inhibitor binding site

McVey WJ, Matthews B, Motley DM, Linse KD, Blass DP, Booth RE, et al. Investigation of the interactions of polyhedral borane anions with serum albumins. J Inorg Biochem. 2008;102(4):943-51. PMID: 18255155. PMAF Usage: MALDI of protein-borane complexes

Morrison AJ, Kim JA, Person MD, Highland J, Xiao J, Wehr TS, et al. Mec1/Tel1 phosphorylation of the INO80 chromatin remodeling complex influences DNA damage checkpoint responses. Cell. 2007;130(3):499-511. PMID: 17693258. PMAF Usage: MALDI protein MW, solution tryptic digest and peptide MS and PSD for phosphopeptide analysis

Zhu F, Xia X, Liu B, Shen J, Hu Y, Person M. IKKalpha shields 14-3-3sigma, a G(2)/M cell cycle checkpoint gene, from hypermethylation, preventing its silencing. Mol Cell. 2007;27(2):214-27. PMID: 17643371. PMAF Usage: MALDI protein ID

Zhang H, White-Phillip JA, Melancon CE, 3rd, Kwon HJ, Yu WL, Liu HW. Elucidation of the kijanimicin gene cluster: insights into the biosynthesis of spirotetronate antibiotics and nitrosugars. J Am Chem Soc. 2007;129(47):14670-83. PMCID: 2515274. PMID: 17985890. PMAF Usage: MALDI of modified sugar

Shen J, Riggs PK, Hensley SC, Schroeder LJ, Traner AR, Kochan KJ, et al. Differential expression of multiple anti-apoptotic proteins in epidermis of IGF-1 transgenic mice as revealed by 2-dimensional gel electrophoresis/mass spectrometry analysis. Mol Carcinog. 2007;46(5):331-40. PMID: 17330866. PMAF Usage: MALDI protein ID

Shen J, Pavone A, Mikulec C, Hensley SC, Traner A, Chang TK, et al. Protein expression profiles in the epidermis of cyclooxygenase-2 transgenic mice by 2-dimensional gel electrophoresis and mass spectrometry. J Proteome Res. 2007;6(1):273-86. PMID: 17203971. PMAF Usage: MALDI protein ID

Lu C, Zhu J, Wang Y, Umeda A, Cowmeadow RB, Lai E, et al. Staphylococcus aureus sortase A exists as a dimeric protein in vitro. Biochemistry. 2007;46(32):9346-54. PMID: 17658894. PMAF Usage: MALDI protein ID and modification analysis of dimer crosslink site

Liu C, Mehdy MC. A nonclassical arabinogalactan protein gene highly expressed in vascular tissues, AGP31, is transcriptionally repressed by methyl jasmonic acid in Arabidopsis. Plant Physiol. 2007;145(3):863-74. PMCID: 2048811. PMID: 17885091. PMAF Usage: SDS-PAGE, in-gel digest, MALDI protein ID

Last Reviewed: February 13, 2012


Maria D. Person
Director

Michelle V. Gadush
Manager

Andre Bui
Technician

John P. O'Brien
Post-doctoral researcher

Delivery Address:

Proteomics Facility
The University of Texas
at Austin
2500 Speedway MBB 1.420
Stop A4800
Austin, TX, USA
78712-1639

Email Address: pmaf
@austin.utexas.edu

Phone:
(512)471-2895

Institute for Cellular and Molecular Biology (ICMB)

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