University of Texas - College of Pharmacy

Protein and Metabolite Analysis Facility

Protein Sequencer

AB Procise 49X cLC Protein Sequencing System

Edman chemistry N-terminal sequencing of proteins and peptides at picomole levels for identification of cleavage sites or homology based protein identification

Recent publications using Procise N-terminal sequencing:

Brune DC, Hampton B, Kobayashi R, Leone JW, Linse KD, Pohl J, et al. ABRF ESRG 2006 study: Edman sequencing as a method for polypeptide quantitation. J Biomol Tech. 2007;18(5):306-20. PMCID: 2392991. PMID: 18166674. PMAF Usage: Procise N-terminal sequencing

Grohman JK, Del Campo M, Bhaskaran H, Tijerina P, Lambowitz AM, Russell R. Probing the mechanisms of DEAD-box proteins as general RNA chaperones: the C-terminal domain of CYT-19 mediates general recognition of RNA. Biochemistry. 2007;46(11):3013-22. PMCID: 2271177. PMID: 17311413. PMAF Usage: Procise N-terminal sequencing identification of enzymatic cleavage sites, Esquire ESI-MS of cleaved fragment

Liu D, Thomas PW, Momb J, Hoang QQ, Petsko GA, Ringe D, et al. Structure and specificity of a quorum-quenching lactonase (AiiB) from Agrobacterium tumefaciens. Biochemistry. 2007;46(42):11789-99. PMID: 17900178. PMAF Usage: Procise N-terminal sequencing of cleaved fusion protein

Lu C, Zhu J, Wang Y, Umeda A, Cowmeadow RB, Lai E, et al. Staphylococcus aureus sortase A exists as a dimeric protein in vitro. Biochemistry. 2007;46(32):9346-54. PMID: 17658894. PMAF Usage: 4700 MALDI-TOF/TOF protein ID and modification analysis of dimer crosslink site, LCQ ESI-MS protein MW, Procise N-terminal sequencing of truncated SrtA protein

Walker JR, Gnanam AJ, Blinkova AL, Hermandson MJ, Karymov MA, Lyubchenko YL, et al. Clostridium taeniosporum spore ribbon-like appendage structure, composition and genes. Mol Microbiol. 2007;63(3):629-43. PMID: 17302797. PMAF Usage: Procise N-terminal sequencing protein ID and SDS-PAGE

Szu PH, Ruszczycky MW, Choi SH, Yan F, Liu HW. Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine. J Am Chem Soc. 2009;131(39):14030-42. PMCID: 2780582. PMID: 19746907. PMAF Usage: Procise N-terminal sequencing protein ID

Thoma RS, Smith JS, Sandoval W, Leone JW, Hunziker P, Hampton B, et al. The ABRF Edman Sequencing Research Group 2008 Study: investigation into homopolymeric amino acid N-terminal sequence tags and their effects on automated Edman degradation. J Biomol Tech. 2009;20(4):216-25. PMCID: 2729483. PMID: 19721823. PMAF Usage: Procise N-terminal sequencing

Be'er A, Ariel G, Kalisman O, Helman Y, Sirota-Madi A, Zhang HP, et al. Lethal protein produced in response to competition between sibling bacterial colonies. Proc Natl Acad Sci U S A. 2010;107(14):6258-63. PMCID: 2851949. PMID: 20308591. PMAF Usage: HPLC separation of peptides, Procise N-terminal sequencing identification of peptides

Hughes RA, Ellington AD. Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA. Nucleic Acids Res. 2010;38(19):6813-30. PMCID: 2965240. PMID: 20571084. PMAF Usage: Procise N-terminal sequencing

Ventura-Suarez A, Cruz-Camarillo R, Rampersad J, Ammons DR, Lopez-Villegas EO, Ibarra JE, et al. Characterization of a novel Bacillus thuringiensis phenotype possessing multiple appendages attached to a parasporal body. Curr Microbiol. 2010;62(1):307-12. PMID: 20640854. PMAF Usage: Procise N-terminal sequencing

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Last Reviewed: January 24, 2011

Maria D. Person
Director

Michelle V. Gadush
MBB Lab Manager

Marvin Mercado
PHR Lab Manager

Chia-Fang Lee
CPRIT post-doctoral fellow

Jacqulyn Tolson,
Research Assistant

Delivery Address:

Protein and Metabolite Analysis Facility
The University of Texas
at Austin
2500 Speedway
Stop A4800
Austin, TX, USA
78712-1639

Email Address: pmaf
@austin.utexas.edu

Phone:
(512)471-2895

Institute for Cellular and Molecular Biology (ICMB)

Center for Research on Environmental Disease (CRED)