Instrumentation available*
The Analytical Instrumentation Facility Core, established in April 1996 in the College of Pharmacy and merged with the ICMB Mass Spectrometry Facility in November 2005 has two primary laboratory spaces located in the Pharmacy Building (PHR 1.110) and the ICMB Building (MBB 1.420). The following instruments are included in the facility:
*Clicking on the names of highlighted instruments will display a photograph of the item.
| Analytical HPLC System | Shimadzu LC-10AS pumps and SIL-10A autosampler with 8 channel UV/VIS Photodiode Array detector, fluorescence detector (RF551) and 4 channel ESA CoulArray electrochemical detector | Analysis of chemicals by absorption, fluorescence and/or electrochemical activity. Analysis of oxidative DNA damage by measuring 8-oxo-deoxyguanosine; xenobiotic metabolites or neurotransmitters, in femtomole to picomole quantity, can be detected. |
| HPLC-ESI-MS/MS System | Michrom Magic 2002 HPLC system with low flow resistive splitter coupled to ThermoFinnigan LCQ ion trap mass spectrometer with ESI, APCI, and microspray interfaces | MS and CID MSn analysis with electrospray and atmospheric chemical ionization. Protein molecular weight, peptide fragmentation and TurboSequest database search for protein identification; macromolecular damage analysis; small molecule detection and characterization. |
| MALDI-MS System | PerSeptive Biosystems Proteomics Solution I: SymBiot I Sample Workstation Voyager-DE PRO | Sample cleanup and target spotting with robot. MALDI-MS and PSD time-of-flight analysis. Automated protein identification from peptide mapping or peptide sequencing and database search. Protein modification analysis, macromolecular damage analysis. |
| GC-MS System | ThermoFinnigan TraceMS GC-quadrupole mass spectrometer with EI, positive and negative CI and selected ion monitoring (SIM) | Analysis of lipophilic biomolecules, fatty acid and metabolite analysis, vitamin and drug quantitative analysis. Enhanced detection sensitivity by SIM to femtomole levels for quantitative analysis. |
| 2D-HPLC System | MicroTech 4 pump 2D HPLC system with flow splitting to achieve flow rates of < 1 mL/min with SCX and reverse phase columns, and UV detector. | Separation of complex mixtures by SCX and HPLC or 2 column HPLC capable of separating peptides, proteins and other biomolecules using capillary flow rates. |
| LC-MALDI-TOF/TOF | LC Packings Ultimate Nano-LC system with Famos autosampler, Switchos and Probot MALDI spotting robot. Applied Biosystems 4700 Proteomics Analyzer MALDI-TOF/TOF utilizing Explorer software with embedded Oracle database. GPS Explorer Workstation with MASCOT database search and Celera Discovery System. | Automated, data and results dependent acquisition of MALDI-MS and MS/MS spectra. Automated protein identification from individual spots and hplc spotted runs in batch mode. HPLC separation of peptides and direct spotting on MALDI target for protein identification of complex mixtures. MS/MS for post-translational modification analysis. Integrated iTRAQ and ICAT analysis software for quantitative proteomics. |
| HPLC-ESI-Triple Quadrupole/Linear Ion Trap | Applied Biosystems 4000 Q Trap LC-MS/MS system with ESI, APCI and nanospray sources coupled with Shimadzu LC-20AD HPLC system (capillary-analytical flow) and LC Packings Ultimate HPLC system (nanoflow). | High-sensitivity LC/MS/MS analysis of post translationally modified (PTM) peptides. Data dependent full-scan MS and MS/MS analysis during precursor ion (PI) and neutral loss (NL) analyses. Multiple reaction monitoring (MRM) and quantitation of small molecules using highly-sensitive triple quadrupole functionality. |
MALDI-TOF for Protein/Peptide MS
MALDI-TOF (2001)
ABI Voyager-DE PRO with reflector detector
•Peptide/protein MW determination, resolution 10,000
Cost: $45/sample, 1/2 price for self-service
HPLC-MALDI-TOF/TOF (2005)
ABI 4700 Proteomics Analyzer
Dionex Ultimate with Probot Spotter
•Protein identification to 30 ng in-gel, 1 ng solution
•Rapid turnaround protein ID
•LC-MALDI spotting for complex samples
•Protein modifications in good relative abundance:
phospho, ubq, methyl, acetyl, ox, inhibitor
Cost: $60/protein ID or MS + MS/MS, $120/LC-MS/MS, 2x for modifications
ESI-MS for Biomolecules
ESI-ion trap (1998)
Michrom HPLC-Thermo LCQ
•Protein molecular weight to 80 kDA
•MS/MS fragmentation of peptides
Cost: $45/MS, $120 for LC-MS/MS
HPLC-ESI-triple quadrupole-ion trap (2007)
ABI 4000 QTRAP + Shimadzu Prominence HPLC
•Quantitative analysis with linear dynamic range O(3-4)
•LOD pg range for many small biomolecules
•Quant. of high and low conc. compounds in same run
•Metabolite characterization
•Low abundance protein targeted identification
Cost: $45/MS, $120 for LC-MS/MS, $225 for new compound method development detection or standard curve
Small Molecule/Metabolite Instruments
Analytical HPLC (1996)
Shimadzu HPLC-UV/Fl-ESA EC
•Oxidative DNA damage by 8-oxo-dG at 30 fmol
•Neurotransmitters
Cost: $10/sample for LC
$15/sample for LC-EC
Gas Chromatography-MS (2001)
Thermo TraceMS
•Quantitative detection of hydrophobic molecules
•LOD pg range for suitable small biomolecules
•Fatty acids/prostaglandins
•4-hydroxynonenal
•Retinoic acid, dicarboxylic acids
Cost: $35/sample
Sample Preparation Services
•protein solution digestion with trypsin
•phosphopeptide enrichment with IMAC
•compound extraction from tissue and serum
Cost: $45/sample
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