University of Texas - College of Pharmacy

Protein and Metabolite Analysis Facility

Resources Chart

The CRED/ICMB Protein and Metabolite Analysis Facility is housed in dedicated instrumentation laboratories located in Pharmacy (PHR 1.110) and at the Institute of Cellular and Molecular Biology (MBB 1.420). The Luminex core is a partnership with Dr. Steven Hursting and is housed in the Dell Pediatric Research Institute. The following instruments are included in the facility with installation date in parantheses.

LC-ESI-MS/MS MBB 1.420 PHR 1.110	Thermo Orbitrap Elite hybrid linear ion trap FT-MS with Dionex Ultimate 3000 nanoflow UPLC (2012) Thermo LTQ Velos Pro dual cell linear ion trap with Proxeon easyLC nanoflow LC (2011) AB Sciex 4000 QTrap hybrid triple quadrupole-linear ion trap with Shimadzu Prominence analytical HPLC (2007)	MS and CID or HCD MSn analysis with electrospray ionization. Protein identification from gel bands, gel sections, and complex mixtures. Proteome Discoverer, X!Tandem, and MASCOT database search engines, Scaffold data visualization, validation, quantitation with spectral counting or stable isotope labeling. Post-translational modification site determination. Protein/peptide MW determination. Multiple reaction monitoring (MRM) quantitation.
MALDI-MS MBB 1.420 PHR 1.110	Applied Biosystems 4700 Proteomics Analyzer MALDI-TOF/TOF (2004) AB Voyager-DE PRO MALDI-TOF (2001)	MALDI-MS and MS/MS for biomolecules. Reflectron detector for peptide molecular weight accuracy of 50 ppm. Linear mode for sensitive detection of proteins. Protein identification of gel bands. Self-service training available for Voyagers.
GC-MS PHR 1.110	ThermoFinnigan TraceMS GC-quadrupole MS (2001)	Analysis of lipophilic biomolecules, fatty acid and metabolite analysis.
LC Systems MBB 1.420 PHR 1.110	Biorad BioLogic DuoFlow FPLC (2 systems-room temp. and 4 C) (2007) Amersham Biosciences AKTA FPLC (1999) Beckman System Gold HPLC (2) (1998) Dionex LC Packings nanoLC (2004)	Self-service and assisted HPLC for purification of biomolecules. Self-service FPLC for high resolution purification of proteins and peptides when recovery of activity is important. Flow rates ul/min-ml/min.
Protein Sequencer MBB 1.420	AB Procise 49X cLC Protein Sequencing	Edman chemistry N-terminal sequencing of proteins and peptides at picomole levels for identification of cleavage sites or homology based protein identification
Peptide Synthesizer MBB 1.420	Rainin Symphony (2001)	12 channel solid-phase peptide synthesizer.
Luminometer MBB 1.420	Berthold Technologies Mithras LB 940 Luminometer (2005)	Measures chemiluminescence in solution for alkaline phosphatase or luciferase assays.
Crystallography Robot Contact Lambowitz lab for use	Art Robbins Instruments Phoenix (2009)	Multiposition, high-throughput liquid-handling platform used for protein crystallography
Gel Electrophoresis MBB 1.420	SDS-PAGE	Training and space provided for self-service gel electrophoresis

Last Reviewed: August 29, 2012