Proteomics Facility

The Proteomics Facility provides services, self-service equipment, and collaborative research for the detection, characterization, and quantification of biomolecules. The facility labs are located in MBB 1.420 (ICMB) and PHR 1.110 (College of Pharmacy). The facility is supported by the Cancer Prevention Research Institute of Texas Shared Instrumentation Award RP110782 and by institutional funds provided by ICMB, UT-Austin and the College of Pharmacy.

News and Announcements:

Call for proposals for proteomics experiments in cancer prevention and research to make use of the Orbitrap Elite mass spectrometer in the University of Texas at Austin Proteomics Facility (PF)

CPRIT grant RP110782 has funded the purchase of the Thermo Orbitrap Elite, a state-of-the art, high-resolution mass spectrometer housed in the ICMB Proteomics Facility. We are calling for proposals on cancer prevention and research to utilize this instrumentation capable of performing qualitative and quantitative proteomics experiments at high sensitivity. Successful applicants will have access to a discounted rate for protein identification services and priority for collaborative quantitation and modification experiments.

Please submit a 1-2 page proposal for consideration, with the following format:

PI, grant agency and number supporting the project, project aims, experimental plan estimating usage requirement and describing preliminary data, and relevance to cancer research, including potential impact.

The proposals will be reviewed by the grant oversight committee and successful applicants will be notified by September 15. The discounted rate will apply to all LC-MS/MS experiments performed by the PF for the approved project in the coming year. Other core services utilized will be billed at the normal rates.

Researchers who are not selected have access to PF proteomics services, either at the internal rates for those working at UT-Austin, or the external rate for other entities. See the website for more information: http://www.utexas.edu/pharmacy/divisions/pharmtox/core/

Submission deadline is July 31, via email to Maria Person

Please visit the Proteomics Facility Wiki at https://wikis.utexas.edu/display/proteomicscore for announcements, protocols and FAQs.




Core Services and Collaborative Projects:

Mass spectrometry:
Protein/peptide molecular weight determination by ESI or MALDI
Protein identification by LC-MS/MS and database search for individual gel bands and complex mixtures
Relative quantitation of peptides/proteins with spectral counting, peak area, or stable isotope labeling by TMT or iTRAQ*
Protein modification analysis for phosphorylation, methylation, acetylation, ubiquitinylation, glycosylation, inhibitor binding, and chemical adduct site determination*

Other MBB lab services/instrumentation:
Edman chemistry N-terminal Sequencing

Self-service instrumentation available:
SDS-PAGE
HPLC and FPLC (4°C or room temperature)
Crystallography robot
Luminometer
MALDI-TOF MS molecular weight determination

When using core services, remember to cite the Proteomics Facility in your publications, and funding sources ES007784 (CRED) and RP110782 (CPRIT) when appropriate, and send the citation to Maria Person. Continued support for the facility depends on documented outcomes.

*If you are interested in collaborating on a protein modification or quantitation project, contact Maria Person.



Last Reviewed: May 28, 2014


Maria D. Person
Director

Michelle V. Gadush
Manager

Andre Bui
Technician


Delivery Address:

Proteomics Facility
The University of Texas
at Austin
2500 Speedway MBB 1.420
Stop A4800
Austin, TX, USA
78712-1639

Email Address: pmaf
@austin.utexas.edu

Phone:
(512)471-2895

Institute for Cellular and Molecular Biology (ICMB)

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