Our lab is interested in understanding how gene expression is regulated across a eukaryotic genome, in normal cells and in disease. Nearly all cells respond to physiological or developmental cues by large-scale transcriptional reprogramming – altering the expression of hundreds to thousands of genes throughout the genome. Such sweeping changes in gene expression also underlie the development of diseases such as cancer, and they can also be caused by normal or abnormal genetic variation between individuals. We focus on gene expression at the level of transcription, regulated by transcription factors and chromatin structure (epigenetics), but are also interested in post-transcriptional regulation mediated by non-coding RNAs. We work in human cells and also use yeast as a model system to address various questions regarding global gene regulation, using genomic and molecular experimental methods that rely heavily on deep sequencing, closely coupled with computational analyses.