In one set of investigations, we attempt to understand the molecular trickery by which the plasmid is able to feed into the chromosome segregation pathway. In another, we explore the molecular details of the copy number amplification pathway. The basic event here is a site-specific DNA recombination event coupled to the replication of the plasmid. We also try to apply the recombination system to bring about directed DNA rearrangements in large genomes-the basic principle of gene targeting and gene therapy.
Affiliated Research Units
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